Negative controls with α-PHP or 4F-α-PVP in KHB with no hepatocytes, and with KHB and hepatocytes with no drugs were being incubated at the same time. Each individual incubation was done when due to the substantial cost of pooled human hepatocytes, but optimistic and negative controls were incorporated to doc https://4fphp16789.blogtov.com/10741574/the-basic-principles-of-4f-php